基于Mn掺杂ZnS量子点-BSA纳米复合材料检测头孢曲松钠

杨茂青; 苗艳明; 闫桂琴

doi:10.3788/fgxb20153604.0472

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基于Mn掺杂ZnS量子点-BSA纳米复合材料检测头孢曲松钠

发光学应用及交叉前沿 | 更新时间：2020-08-12

- 基于Mn掺杂ZnS量子点-BSA纳米复合材料检测头孢曲松钠
- Detection of Ceftriaxone Based on ZnS:Mn Quantum Dots-BSA Nanohybrids
- 发光学报 2015年36卷第4期页码：472-479
- 作者机构：
  
  山西师范大学生命学院,山西临汾,041000
- 作者简介：
- 基金信息：
  
  山西省重点化学优势学科建设项目(912019)();国家教育部博士点联合基金(20111404110002)资助项目()
- DOI：10.3788/fgxb20153604.0472
  中图分类号： O657;O482.31
- 收稿：2015-01-28，
  
  修回：2015-2-22，
  
  纸质出版：2015-04-03
- 稿件说明：
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杨茂青, 苗艳明, 闫桂琴. 基于Mn掺杂ZnS量子点-BSA纳米复合材料检测头孢曲松钠[J]. 发光学报, 2015,36(4): 472-479

YANG Mao-qing, MIAO Yan-ming, YAN Gui-qin. Detection of Ceftriaxone Based on ZnS:Mn Quantum Dots-BSA Nanohybrids[J]. Chinese Journal of Luminescence, 2015,36(4): 472-479
杨茂青, 苗艳明, 闫桂琴. 基于Mn掺杂ZnS量子点-BSA纳米复合材料检测头孢曲松钠[J]. 发光学报, 2015,36(4): 472-479 DOI： 10.3788/fgxb20153604.0472.

YANG Mao-qing, MIAO Yan-ming, YAN Gui-qin. Detection of Ceftriaxone Based on ZnS:Mn Quantum Dots-BSA Nanohybrids[J]. Chinese Journal of Luminescence, 2015,36(4): 472-479 DOI： 10.3788/fgxb20153604.0472.

摘要

以3-巯基丙酸(MPA)为稳定剂

采用水相合成法合成了具有优良光学性质的Mn掺杂ZnS量子点。该量子点在室温条件下即可发射较强的磷光信号。将量子点与牛血清蛋白(BSA)偶联后

形成了纳米复合材料。由于BSA对量子点表面缺陷进行了有效修复

量子点发出的磷光明显增强。当加入头孢曲松钠后

头孢曲松钠与BSA的相互作用使量子点的磷光被有效猝灭

该磷光猝灭量(△

)与头孢曲松钠的浓度在一定范围内呈良好的线性关系(

=0.99)。量子点与BSA偶联的最佳条件为:pH=7.4

反应温度37 ℃

反应时间40 min

BSA浓度80 mgL

-1

量子点浓度40 mgL

-1

。反应形成新的生物纳米复合材料

作为头孢曲松钠的磷光探针

其线性范围为0~30 molL

-1

Abstract

Using 3-mercaptopropionic acid(MPA) as the stabilizer

ZnS:Mn quantum dots(QDs) were synthesized

via

water phase method

which could emit strong phosphorescence at room temperature. Bovine serum albumin (BSA) can effectively recover the defect on the surface of ZnS:Mn QDs

so the phosphorescence intensity of ZnS:Mn QDs can be enhanced after the conjugation between BSA and ZnS:Mn QDs. Ceftriaxone sodium can obviously quench the phosphorescence of ZnS:Mn QDs-BSA

and the quenched phosphorescence intensity amount (△

) is linearly proportional to the concentration of ceftriaxone sodium with the correlation coefficient

=0.99. The optimal condition of conjunction between ZnS:Mn QDs and BSA is as following: pH=7.4

reaction temperature of 37 ℃

response time of 40 min

BSA concentration of 80 mgL

-1

ZnS:Mn QDs concentration of 40 mgL

-1

respectively. As the phosphorescence probe

the synthesized ZnS:Mn QDs-BSA can detect ceftriaxone sodium effectively with the linear scope of 0－30 molL

-1

correlation coefficient

=0.99

inspection limit of 0.14 molL

-1

relative standard deviation of 1.63%

respectively.

关键词

Keywords

references

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